| First Author | Yamagami T | Year | 1999 |
| Journal | Immunity | Volume | 11 |
| Issue | 3 | Pages | 309-16 |
| PubMed ID | 10514009 | Mgi Jnum | J:57910 |
| Mgi Id | MGI:1345940 | Doi | 10.1016/s1074-7613(00)80106-5 |
| Citation | Yamagami T, et al. (1999) Four of five RAG-expressing JCkappa-/- small pre-BII cells have no L chain gene rearrangements: detection by high-efficiency single cell PCR. Immunity 11(3):309-16 |
| abstractText | Single cell PCR assays have been further developed that detect over 80% of all VkappaJkappa, VkappaRS, and VlambdaJlambda rearrangements at efficiencies between 70% and 90%. These IgL chain gene rearrangement assays were used with small pre-BII cells that develop in comparably high numbers in the bone marrow of wild-type, Ckappa-deficient, and JCkappa-deficient homozygous and heterozygous mice. In all of these mice, only 15%-25% of all small pre-BII cells carry VlambdaJlambda rearrangements. These results confirm that lambdaL chain gene rearrangements occur independently of kappaL chain gene rearrangement and expression. They also show that a large part of the small pre-BII cells that express the rearrangement machinery can develop without IgL chain gene rearrangements. |
