| First Author | Reue K | Year | 1993 |
| Journal | J Lipid Res | Volume | 34 |
| Issue | 6 | Pages | 893-903 |
| PubMed ID | 8354955 | Mgi Jnum | J:12614 |
| Mgi Id | MGI:60855 | Citation | Reue K, et al. (1993) Genetic variation in mouse apolipoprotein A-IV expression is determined pre- and post-transcriptionally. J Lipid Res 34(6):893-903 |
| abstractText | Among inbred mouse strains there is a striking genetic variation in the levels of apolipoprotein A-IV (apoA-IV) mRNA in the liver, although intestinal mRNA levels vary only twofold in these strains. In the present study we have characterized the apoA-IV expression phenotypes in strains C57BL/6J and 129/J, and investigated the molecular basis for the genetic variation. We report that the two strains differ eight- to tenfold both in the levels of apoA-IV mRNA and in the rate of apoA-IV protein synthesis in liver. Presumably due to the increased synthetic rate, strain 129 exhibits a threefold higher concentration of apoA-IV protein in the circulation. mRNA synthesis and turnover studies indicate that both transcriptional and post-transcriptional events contribute to the genetic variation in steady state apoA-IV mRNA levels. An analysis of the levels of apoA-IV mRNA derived from 129 and C57BL/6 alleles in F1 mice indicates that the genetic control of apoA-IV mRNA levels involves both cis-acting elements linked to the apoA-IV gene, and genetically distinct trans-acting factors. |
