| First Author | Zarrin AA | Year | 2007 |
| Journal | Science | Volume | 315 |
| Issue | 5810 | Pages | 377-81 |
| PubMed ID | 17170253 | Mgi Jnum | J:220885 |
| Mgi Id | MGI:5636831 | Doi | 10.1126/science.1136386 |
| Citation | Zarrin AA, et al. (2007) Antibody class switching mediated by yeast endonuclease-generated DNA breaks. Science 315(5810):377-81 |
| abstractText | Antibody class switching in activated B cells uses class switch recombination (CSR), which joins activation-induced cytidine deaminase (AID)-dependent double-strand breaks (DSBs) within two large immunoglobulin heavy chain (IgH) locus switch (S) regions that lie up to 200 kilobases apart. To test postulated roles of S regions and AID in CSR, we generated mutant B cells in which donor Smu and accepter Sgamma1 regions were replaced with yeast I-SceI endonuclease sites. We found that site-specific I-SceI DSBs mediate recombinational IgH locus class switching from IgM to IgG1 without S regions or AID. We propose that CSR evolved to exploit a general DNA repair process that promotes joining of widely separated DSBs within a chromosome. |
