| First Author | Ohkusu-Tsukada K | Year | 2019 |
| Journal | Genes Immun | Volume | 20 |
| Issue | 1 | Pages | 74-81 |
| PubMed ID | 29282355 | Mgi Jnum | J:273488 |
| Mgi Id | MGI:6293965 | Doi | 10.1038/s41435-017-0003-y |
| Citation | Ohkusu-Tsukada K, et al. (2019) Low expression of a D(dm7)/L(dm7)-hybrid mutant (D/L(dm7)) in the novel haplotype H-2(nc) identified in atopic dermatitis model NC/Nga mice. Genes Immun 20(1):74-81 |
| abstractText | Environmental factors and the major histocompatibility complex (MHC) are involved in the pathogenesis of atopic dermatitis (AD). However, MHC type (H2 haplotype) of AD model mice NC/Nga is poorly understood. Alloreactive CD8(+) or CD4(+) T cells in NC/Nga strongly responded to each antigen-presenting cells (A/J: H-2(a), C57BL/6: H-2(b), BALB/c: H-2(d), or C3H/HeJ: H-2(k)), suggesting that NC/Nga has other H2 haplotype. Polymorphic microsatellite (CA)(n) repeats in TNF-alpha gene differ based on the H2 haplotype at present. NC/Nga's (CA)(n) repeats (n = 19) were different from other examined strains, A/J (n = 14), BALB/c (n = 14), C3H/HeJ (n = 16), and C57BL/6 (n = 20). Using flow cytometry and genotyping, we demonstrated the NC/Nga H2 haplotype had a unique phenotype (K(d), I-A(k), and I-E(k)) in which D(d) and L(d) lacked as protein despite sensitive mRNA detection. The loss of D(d) and L(d) was caused by forming a unique D(dm7)/L(dm7)-hybrid mutant (D/L(dm7)). We propose to call this novel H2 haplotype the "H-2(nc)," and provide the important information regarding the AD research using NC/Nga mice. |
