| First Author | Kumano K | Year | 2000 |
| Journal | Biochem Biophys Res Commun | Volume | 270 |
| Issue | 1 | Pages | 209-14 |
| PubMed ID | 10733929 | Mgi Jnum | J:61476 |
| Mgi Id | MGI:1355030 | Doi | 10.1006/bbrc.2000.2403 |
| Citation | Kumano K, et al. (2000) Blockade of JAK2 by tyrphostin AG-490 inhibits antigen-induced eosinophil recruitment into the mouse airways. Biochem Biophys Res Commun 270(1):209-14 |
| abstractText | We studied the effect of tyrphostin AG-490, a specific Janus kinase 2 (JAK2) inhibitor, on antigen-induced eosinophil recruitment into the airways of sensitized mice and on IL-5-induced chemokinesis and adhesiveness of eosinophils. The in vivo administration of AG-490 prevented antigen-induced eosinophil infiltration in the airways of sensitized mice in a dose-dependent manner. However, the administration of AG-490 did not affect antigen-induced IL-5 production in the airways nor in vitro antigen-induced IL-5 production and T cell proliferation of spleen cells. Furthermore, AG-490 inhibited IL-5-induced chemokinesis and beta1-integrin adhesiveness of eosinophils in vitro. Because antigen-induced eosinophil recruitment into the airways is mediated by IL-5, these results indicate that JAK2 activation is critical for antigen-induced, IL-5-dependent mobilization of eosinophils into the tissue. Copyright 2000 Academic Press. |
