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Allele : Col1a1<tm2(tetO-GFP/RNAi:Kras462)Ttam> collagen, type I, alpha 1; targeted mutation 2, Tuomas Tammela
Primary Identifier  MGI:7610034 Allele Type  Targeted
Attribute String  Inducible, Knockdown, Reporter, RMCE-ready Gene  Col1a1
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
molecularNote  The targeting vector was designed to insert a short hairpin RNA (shRNA) targeting Kras fused to green fluorescent protein (GFP) cDNA, placed downstream of a tetracycline-responsive element (TRE) promoter, and targeted via recombinase-mediated cassette exchange (RMCE) into the ubiquitously expressed collagen, type I, alpha 1 (Col1a1) gene. shKras462 indicates that the position of the first base pair bound by the shRNA seed sequence is 462 in the KRAS transcript. The targeting vector also contained a hygromycin resistance cassette.
  • mutations:
  • Insertion
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Trail: Allele

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0 Mutation Involves

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