| Primary Identifier | MGI:4999797 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Nek7 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Most of exons 3 and 4 of the gene is replaced with b-galactosidase (b-gal)-Neo resistance cassette, which deletes 1630 bp and insert premature termination of Nek7 coding at amino-acid 75 but leaving intact the splice acceptor site of exon 3 and the splice donor site of exon 4. Western blot confirmed absence of protein product in cultured MEF. This is a null allele. |
