| First Author | Bethel CR | Year | 1994 |
| Journal | Biochem Mol Biol Int | Volume | 34 |
| Issue | 2 | Pages | 385-92 |
| PubMed ID | 7531538 | Mgi Jnum | J:21269 |
| Mgi Id | MGI:69290 | Citation | Bethel CR, et al. (1994) Molecular cloning of mouse insulin-like growth factor binding protein 4 (IGFBP4) cDNA and expression of a fusion protein with IGF-binding activity. Biochem Mol Biol Int 34(2):385-92 |
| abstractText | The TC-1 bone marrow stromal cell line expresses a 2.3 kb IGFBP-4 mRNA transcript. Reverse transcription/polymerase chain reaction was used to amplify the complete open reading frame of the insulin-like growth factor binding protein-4 (IGFBP-4) from poly(A)+ of a murine bone marrow stromal cell line (TC-1). Sequence analysis reveals that the murine IGFBP-4 is highly homologous to the rat IGFBP-4 and less so to the human IGFBP-4. The inferred amino acid sequence has a molecular weight of 25.7 kD. An IGFBP-4/maltose binding protein fusion peptide expression in the pMal-p2 vector produced a fusion protein exhibiting both IGFBP immunoreactivity and IGF-I binding activity with specificity characteristic of IGFBPs. |
