| First Author | Ramakrishnan SN | Year | 2009 |
| Journal | Biochem Biophys Res Commun | Volume | 388 |
| Issue | 4 | Pages | 654-9 |
| PubMed ID | 19682428 | Mgi Jnum | J:203405 |
| Mgi Id | MGI:5526936 | Doi | 10.1016/j.bbrc.2009.08.045 |
| Citation | Ramakrishnan SN, et al. (2009) Rev-erb beta regulates the Srebp-1c promoter and mRNA expression in skeletal muscle cells. Biochem Biophys Res Commun 388(4):654-9 |
| abstractText | The nuclear hormone receptor, Rev-erb beta operates as a transcriptional silencer. We previously demonstrated that exogenous expression of Rev-erb betaDeltaE in skeletal muscle cells increased Srebp-1c mRNA expression. We validated these in vitro observations by injection of an expression vector driving Rev-erb betaDeltaE expression into mouse tibialis muscle that resulted in increased Srebp-1c mRNA expression. Paradoxically, Rev-erb beta siRNA expression in skeletal muscle cells repressed Srebp-1c expression, and indicated that Rev-erb beta expression was necessary for Srebp-1c expression. ChIP analysis demonstrated that Rev-erb beta was recruited to the Srebp-1c promoter. Moreover, Rev-erb beta trans-activated the Srebp-1c promoter, in contrast, Rev-erb beta efficiently repressed the Rev-erb alpha promoter, a previously characterized target gene. Finally, treatment with the Rev-erb agonist (hemin) (i) increased the trans-activation of the Srebp-1c promoter by Rev-erb beta; and (ii) increased Rev-erb beta and Srebp-1c mRNA expression. These data suggest that Rev-erb beta has the potential to activate gene expression, and is a positive regulator of Srebp-1c, a regulator of lipogenesis. |
