Primary Identifier | MGI:5498678 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Aurka |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 2. An additional loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the neomycin resistance cassette and left exon 2 floxed. Cre-mediated recombination removed exon 2. Western blot analysis confirmed the absence of protein expression. |