| First Author | Zambrowicz BP | Year | 1993 |
| Journal | Proc Natl Acad Sci U S A | Volume | 90 |
| Issue | 11 | Pages | 5071-5 |
| PubMed ID | 8389466 | Mgi Jnum | J:12578 |
| Mgi Id | MGI:60819 | Doi | 10.1073/pnas.90.11.5071 |
| Citation | Zambrowicz BP, et al. (1993) Analysis of the mouse protamine 1 promoter in transgenic mice. Proc Natl Acad Sci U S A 90(11):5071-5 |
| abstractText | Protamines are small arginine-rich proteins that package DNA in spermatozoa. The mouse protamine 1 (Prm-1) gene is transcribed exclusively in post-meiotic spermatids. To identify elements in the Prm-1 promoter required for spermatid-specific transcription, we generated transgenic mice by microinjection of transgenes containing Prm-1 5' flanking sequences with 5' truncations or internal deletions of conserved sequences linked to a marked Prm-1 gene. We also tested Prm-1 promoter regions with a heterologous human growth hormone reporter gene. We conclude that a 113-bp region can direct spermatid-specific transcription and we have defined sequences within this region that are essential for proper function. These results will facilitate the isolation and characterization of transcription factors essential for post-meiotic gene expression. |
