| First Author | Tate ML | Year | 1995 |
| Journal | Genetics | Volume | 139 |
| Issue | 3 | Pages | 1383-91 |
| PubMed ID | 7768446 | Mgi Jnum | J:23265 |
| Mgi Id | MGI:71031 | Doi | 10.1093/genetics/139.3.1383 |
| Citation | Tate ML, et al. (1995) A new gene mapping resource: interspecies hybrids between Pere David's deer (Elaphurus davidianus) and red deer (Cervus elaphus). Genetics 139(3):1383-91 |
| abstractText | Three male F1 hybrids between Pere David's deer and red deer were mated to red deer to produce 143 backcross calves. The pedigrees are a rare example of a fertile hybrid between evolutionarily divergent species. We examined the use of these families for genetic mapping of evolutionarily conserved (Type I) loci by testing for genetic linkage between five species-specific protein variants and 12 conserved DNA probes. Two probes were homologous, and the remainder syntenic, to the protein coding loci in cattle or humans. Using six restriction enzymes, each DNA probe detected one or more restriction fragments specific to Pere David's deer. Linkage analyses among the species-specific variants placed the loci into four linkage groups within which linkage between adjacent loci and gene order was supported by a LOD > 3. The linkage groups were (HPX, HBB)-FSHB-ACP2, LDHA-CD5-IGF2, BMP3-(GC, ALB)-(KIT, PDGFRA) and LDLR-C3-FGF1. Southern and protein analysis of LDHA and ALB provided identical segregation data. These linkage groups were consistent with the cattle gene map and provide new information for comparing the gene maps of ruminants, humans and mice. The deer hybrids are an important new resource that can contribute to the comparative analysis of the mammalian genome. |
