| First Author | Klemsz MJ | Year | 1990 |
| Journal | Cell | Volume | 61 |
| Issue | 1 | Pages | 113-24 |
| PubMed ID | 2180582 | Mgi Jnum | J:10374 |
| Mgi Id | MGI:58826 | Doi | 10.1016/0092-8674(90)90219-5 |
| Citation | Klemsz MJ, et al. (1990) The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene [see comments]. Cell 61(1):113-24 |
| abstractText | We have isolated a cDNA clone, PU.1, that codes for a new tissue-specific DNA binding protein. Analysis of the binding site by methylation interference and DNAase 1 protection revealed that the PU.1 protein recognized a purine-rich sequence, 5'-GAGGAA-3' (PU box). The PU.1 protein was shown to be a transcriptional activator that is expressed in macrophages and B cells. cDNA constructions used to generate proteins lacking portions of either the amino- or carboxy-terminal ends of the PU.1 protein placed the DNA binding domain in the highly basic carboxy-terminal domain of the protein. The amino acid sequence in the binding domain of PU.1 has considerable identity with proteins belonging to the ets oncogene family. |
