| First Author | Lénárt K | Year | 2020 |
| Journal | Int J Mol Sci | Volume | 21 |
| Issue | 11 | PubMed ID | 32485850 |
| Mgi Jnum | J:302413 | Mgi Id | MGI:6508262 |
| Doi | 10.3390/ijms21113865 | Citation | Lenart K, et al. (2020) Transglutaminase 2 Has Metabolic and Vascular Regulatory Functions Revealed by In Vivo Activation of Alpha1-Adrenergic Receptor. Int J Mol Sci 21(11):3865 |
| abstractText | The multifunctional tissue transglutaminase has been demonstrated to act as alpha1-adrenergic receptor-coupled G protein with GTPase activity in several cell types. To explore further the pathophysiological significance of this function we investigated the in vivo effects of the alpha1-adrenergic receptor agonist phenylephrine comparing responses in wild type and TG2(-/-) mice. Injection of phenylephrine, but not a beta3-adrenergic agonist (CL-316,243), resulted in the long-term decline of the respiratory exchange ratio and lower lactate concentration in TG2(-/-) mice indicating they preferred to utilize fatty acids instead of glucose as fuels. Measurement of tail blood pressure revealed that the vasoconstrictive effect of phenylephrine was milder in TG2(-/-) mice leading to lower levels of lactate dehydrogenase (LDH) isoenzymes in blood. LDH isoenzyme patterns indicated more damage in lung, liver, kidney, skeletal, and cardiac muscle of wild type mice; the latter was confirmed by a higher level of heart-specific CK-MB. Our data suggest that TG2 as an alpha1-adrenergic receptor-coupled G protein has important regulatory functions in alpha1-adrenergic receptor-mediated metabolic processes and vascular functions. |
