| First Author | Liu GH | Year | 2009 |
| Journal | PLoS One | Volume | 4 |
| Issue | 9 | Pages | e7031 |
| PubMed ID | 19753306 | Mgi Jnum | J:159054 |
| Mgi Id | MGI:4441113 | Doi | 10.1371/journal.pone.0007031 |
| Citation | Liu GH, et al. (2009) Sumoylation regulates nuclear localization of lipin-1alpha in neuronal cells. PLoS One 4(9):e7031 |
| abstractText | Lipin-1 is a protein that has dual functions as a phosphatidic acid phosphohydrolase (PAP) and a nuclear transcriptional coactivator. It remains unknown how the nuclear localization and coactivator functions of lipin-1 are regulated. Here, we show that lipin-1 (including both the alpha and beta isoforms) is modified by sumoylation at two consensus sumoylation sites. We are unable to detect sumoylation of the related proteins lipin-2 and lipin-3. Lipin-1 is sumoylated at relatively high levels in brain, where lipin-1alpha is the predominant form. In cultured embryonic cortical neurons and SH-SY5Y neuronal cells, ectopically expressed lipin-1alpha is localized in both the nucleus and the cytoplasm, and the nuclear localization is abrogated by mutating the consensus sumyolation motifs. The sumoylation site mutant of lipin-1alpha loses the capacity to coactivate the transcriptional (co-) activators PGC-1alpha and MEF2, consistent with its nuclear exclusion. Thus, these results show that sumoylation facilitates the nuclear localization and transcriptional coactivator behavior of lipin-1alpha that we observe in cultured neuronal cells, and suggest that lipin-1alpha may act as a sumoylation-regulated transcriptional coactivator in brain. |
