| First Author | Krautwurst D | Year | 1998 |
| Journal | Cell | Volume | 95 |
| Issue | 7 | Pages | 917-26 |
| PubMed ID | 9875846 | Mgi Jnum | J:51606 |
| Mgi Id | MGI:1321337 | Doi | 10.1016/s0092-8674(00)81716-x |
| Citation | Krautwurst D, et al. (1998) Identification of ligands for olfactory receptors by functional expression of a receptor library. Cell 95(7):917-26 |
| abstractText | The recognition of odorants by olfactory receptors represents the first stage in odor discrimination. Here, we report the generation of an expression library containing a large and diverse repertoire of mouse olfactory receptor sequences in the transmembrane II-VII region. From this library, 80 chimeric receptors were tested against 26 odorants after transfection into HEK-293 cells. Three receptors were identified to respond to micromolecular concentrations of carvone, (-) citronellal, and limonene, respectively. We also found that the mouse I7 receptor, unlike the rat I7 receptor, prefers heptanal instead of octanal, as a result of a single valine-to-isoleucine substitution. This finding represents the beginning of a molecular understanding of odorant recognition. The identification, on a large scale, of cognate receptor-odorant interactions should provide insight into olfactory coding mechanisms. |
