| First Author | Ikeda A | Year | 2007 |
| Journal | Biochem Biophys Res Commun | Volume | 363 |
| Issue | 3 | Pages | 835-9 |
| PubMed ID | 17904530 | Mgi Jnum | J:127350 |
| Mgi Id | MGI:3763599 | Doi | 10.1016/j.bbrc.2007.09.062 |
| Citation | Ikeda A, et al. (2007) Abnormal features in mutant cerebellar Purkinje cells lacking junctophilins. Biochem Biophys Res Commun 363(3):835-9 |
| abstractText | Junctional membrane complexes (JMCs) generated by junctophilins are required for Ca(2+)-mediated communication between cell-surface and intracellular channels in excitable cells. Knockout mice lacking neural junctophilins (JP-DKO) show severe motor defects and irregular cerebellar plasticity due to abolished channel crosstalk in Purkinje cells (PCs). To precisely understand aberrations in JP-DKO mice, we further analyzed the mutant PCs. During the induction of cerebellar plasticity via electrical stimuli, JP-DKO PCs showed insufficient depolarizing responses. Immunochemistry detected mild impairment in synaptic maturation and hyperphosphorylation of protein kinase Cgamma in JP-DKO PCs. Moreover, gene expression was slightly altered in the JP-DKO cerebellum. Therefore, the mutant PCs bear marginal but widespread abnormalities, all of which likely cause cerebellar motor defects in JP-DKO mice. |
