| Primary Identifier | MGI:5605399 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ift27 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Mutant cell line HEPD0653_7_E10 was used to create mutant mice which demonstrated germline transmission. |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 78049209 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 78051786. The critical exon(s) is/are thus flanked by loxP sites. The "conditional ready" (floxed) allele created by flp recombinase expression is further modified by subsequent cre expression to create a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. |
