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Allele : Sptlc1<em2Rwb> serine palmitoyltransferase, long chain base subunit 1; endonuclease-mediated mutation 2, Robert W Burgess
Primary Identifier  MGI:7427573 Allele Type  Endonuclease-mediated
Attribute String  Humanized sequence Gene  Sptlc1
Strain of Origin  C57BL/6J Is Recombinase  false
Is Wild Type  false
molecularNote  CRISPR/cas9 endonuclease-mediated genome editing is used to create an cysteine to tryptophan substitution at position 133 (C133W, TGT to TGG) and a silent C to G mutation in codon 132 (T132T) in exon 5. The silent mutation eliminates the PAM site and prevents recutting with Cas9.
  • mutations:
  • Nucleotide substitutions
  • synonyms:
  • Sptlc1<C133W>,
  • Sptlc1<C133W>
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Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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Trail: Allele




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