Primary Identifier | MGI:3575311 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Sh2d1a |
Transmission | Germline | Strain of Origin | 129S2/SvPas |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A neomycin resistance gene replaced a sequence consisting of 606 bp of intron 1 and 25 bp of exon 2. RT-PCR and sequencing showed that the resulting transcript skipped exon 2, and therefore contained a 64 bp frame-shift deletion. Western blot demonstrated the lack of protein in mutant thymocytes. |