| First Author | Candreva T | Year | 2019 |
| Journal | Clin Sci (Lond) | Volume | 133 |
| Issue | 22 | Pages | 2345-2360 |
| PubMed ID | 31722009 | Mgi Jnum | J:289749 |
| Mgi Id | MGI:6433004 | Doi | 10.1042/CS20190753 |
| Citation | Candreva T, et al. (2019) Docosahexaenoic acid slows inflammation resolution and impairs the quality of healed skin tissue. Clin Sci (Lond) 133(22):2345-2360 |
| abstractText | There is no consensus on the effects of omega-3 (omega-3) fatty acids (FA) on cutaneous repair. To solve this problem, we used 2 different approaches: (1) FAT-1 transgenic mice, capable of producing endogenous omega-3 FA; (2) wild-type (WT) mice orally supplemented with DHA-enriched fish oil. FAT-1 mice had higher systemic (serum) and local (skin tissue) omega-3 FA levels, mainly docosahexaenoic acid (DHA), in comparison with WT mice. FAT-1 mice had increased myeloperoxidase (MPO) activity and content of CXCL-1 and CXCL-2, and reduced IL-10 in the skin wound tissue three days after the wound induction. Inflammation was maintained by an elevated TNF-alpha concentration and presence of inflammatory cells and edema. Neutrophils and macrophages, isolated from FAT-1 mice, also produced increased TNF-alpha and reduced IL-10 levels. In these mice, the wound closure was delayed, with a wound area 6-fold bigger in relation with WT group, on the last day of analysis (14 days post-wounding). This was associated with poor orientation of collagen fibers and structural aspects in repaired tissue. Similarly, DHA group had a delay during late inflammatory phase. This group had increased TNF-alpha content and CD45+F4/80+ cells at the third day after skin wounding and increased concentrations of important metabolites derived from omega-3, like 18-HEPE, and reduced concentrations of those from omega-6 FA. In conclusion, elevated DHA content, achieved in both FAT-1 and DHA groups, slowed inflammation resolution and impaired the quality of healed skin tissue. |
