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Allele : Gt(ROSA)26Sor<tm10.1(CAG-birA*,-mKate2)Amc> gene trap ROSA 26, Philippe Soriano; targeted mutation 10.1, Andrew P McMahon
Primary Identifier  MGI:7279067 Allele Type  Targeted
Attribute String  Conditional ready, Reporter Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  B6(Cg)-Tyr<c-2J>/J
Is Recombinase  false Is Wild Type  false
molecularNote  The targeting vector is designed with, from 5' to 3', a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a floxed cassette containing a green fluorescent protein (GFP) sequence and a polyadenylation signal, a myc-tagged endoplasmic reticulum-directed promiscuous biotin ligase sequence (BirA*G3-ER), an internal ribosome entry site (IRES), and an mKate2 red fluorescent protein sequence. This targeting vector, which also includes an attB-neoR-attP cassette, was inserted into the Gt(ROSA)26Sor gene. PhiC31o-mediated recombination removed the neo cassette.
  • mutations:
  • Insertion
  • synonyms:
  • R26 BirA*G3-ER,
  • R26 BirA*G3-ER
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