| First Author | Ro HS | Year | 2007 |
| Journal | Obesity (Silver Spring) | Volume | 15 |
| Issue | 2 | Pages | 288-302 |
| PubMed ID | 17299101 | Mgi Jnum | J:125333 |
| Mgi Id | MGI:3758181 | Doi | 10.1038/oby.2007.569 |
| Citation | Ro HS, et al. (2007) Adipocyte enhancer-binding protein 1 modulates adiposity and energy homeostasis. Obesity (Silver Spring) 15(2):288-302 |
| abstractText | OBJECTIVE: To determine whether adipocyte enhancer binding protein (AEBP) 1, a transcriptional repressor that is down-regulated during adipogenesis, functions as a critical regulator of adipose tissue homeostasis through modulation of phosphatase and tensin homolog deleted on chromosome ten (PTEN) tumor suppressor activity and mitogen-activated protein kinase (MAPK) activation. RESEARCH METHODS AND PROCEDURES: We examined whether AEBP1 physically interacts with PTEN in 3T3-L1 cells by coimmunoprecipitation analysis. We generated AEBP1-null mice and examined the physiological role of AEBP1 as a key modulator of in vivo adiposity. Using adipose tissue from wild-type and AEBP1-null animals, we examined whether AEBP1 affects PTEN protein level. RESULTS: AEBP1 interacts with PTEN, and deficiency of AEBP1 increases adipose tissue PTEN mass. AEBP1-null mice have reduced adipose tissue mass and enhanced apoptosis with suppressed survival signal. Primary pre-adipocytes from AEBP1-null adipose tissues exhibit lower basal MAPK activity with defective proliferative potential. AEBP1-null mice are also resistant to diet-induced obesity, suggesting a regulatory role for AEBP1 in energy homeostasis. DISCUSSION: Our results suggest that AEBP1 negatively regulates adipose tissue PTEN levels, in conjunction with its role in proliferation and differentiation of pre-adipocytes, as a key functional role in modulation of in vivo adiposity. |
