| First Author | Segade F | Year | 1995 |
| Journal | J Immunol | Volume | 154 |
| Issue | 5 | Pages | 2384-92 |
| PubMed ID | 7868905 | Mgi Jnum | J:22946 |
| Mgi Id | MGI:70815 | Doi | 10.4049/jimmunol.154.5.2384 |
| Citation | Segade F, et al. (1995) Isolation of nine gene sequences induced by silica in murine macrophages. J Immunol 154(5):2384-92 |
| abstractText | Macrophage activation by silica is the initial step in the development of silicosis. To identify genes that might be involved in silica-mediated activation, RAW 264.7 mouse macrophages were treated with silica for 48 h, and a subtracted cDNA library enriched for silica-induced genes (SIG) was constructed and differentially screened. Nine cDNA clones (designated SIG-12, -14, -20, -41, -61, -81, -91, -92, and -111) were partially sequenced and compared with sequences in GenBank/EMBL databases. SIG-12, -14, and -20 corresponded to the genes for ribosomal proteins L13a, L32, and L26, respectively. SIG-61 is the mouse homologue of p21 RhoC. SIG-91 is identical to the 67-kDa high-affinity laminin receptor. Four genes were not identified and are novel. All of the mRNAs corresponding to the nine cloned cDNAs were inducible by silica. Steady-state levels of mRNAs in RAW 264.7 cells treated with various macrophage activators and inducers of signal transduction pathways were determined. A complex pattern of induction and repression was found, indicating that upon phagocytosis of silica particles, many regulatory mechanisms of gene expression are simultaneously triggered. |
