| First Author | Dalal I | Year | 1998 |
| Journal | Blood | Volume | 91 |
| Issue | 3 | Pages | 844-51 |
| PubMed ID | 9446644 | Mgi Jnum | J:45713 |
| Mgi Id | MGI:1195868 | Doi | 10.1182/blood.v91.3.844.844_844_851 |
| Citation | Dalal I, et al. (1998) Cloning and characterization of the human homolog of mouse Jak2. Blood 91(3):844-51 |
| abstractText | Members of the Jak family play a critical role in signal transduction mediated by cytokine and hormone receptors. In this study, we report the cloning and characterization of human Jak2. The predicted amino acid sequence shows 91% homology to the described murine Jak2, but with a significant difference in the extreme C-terminal sequence. Using the human cDNA as a probe, we localized the gene for human Jak2 to chromosome 9p23-24, Human Jak2 mRNA is highly expressed in the spleen, lymph nodes, and peripheral blood lymphocytes (PBLs). A polyclonal antibody raised against the unique C-terminus of human Jak2 was used to characterize Jak2 protein. Levels of Jak2 protein expression increased significantly in mitogen-and anti-IgM- stimulated B cells and to a lesser degree in activated T cells. In addition, high levels of Jak2 protein were detected in pre-B leukemia cells, (C) 1998 by The American Society of Hematology. |
