| First Author | Ruetz S | Year | 1994 |
| Journal | Cell | Volume | 77 |
| Issue | 7 | Pages | 1071-81 |
| PubMed ID | 7912658 | Mgi Jnum | J:18957 |
| Mgi Id | MGI:67189 | Doi | 10.1016/0092-8674(94)90446-4 |
| Citation | Ruetz S, et al. (1994) Phosphatidylcholine translocase: a physiological role for the mdr2 gene. Cell 77(7):1071-81 |
| abstractText | P-glycoproteins (P-gps) encoded by the mouse mdr2 and mdr3 genes were expressed in secretory vesicles (SVs) from the yeast mutant sec6-4, and their capacity to function as a lipid translocase/flippase was tested. An assay that uses a fluorescent phosphatidylcholine (PC) analog was developed to quantitate asymmetric lipid distribution in the outer and inner leaflets of the lipid bilayer of these vesicles. Mdr2 expression in SVs caused a time- and temperature-dependent enhancement of PC translocation to the inner leaflet of the membrane. The Mdr2-mediated effect was specific since expression of Mdr3 in these vesicles was without effect on the membrane distribution of PC. Increased Mdr2-mediated PC translocation was strictly ATP and Mg2+ dependent, was abrogated by the ATPase inhibitor vanadate and the P-gp modulator verapamil, but was insensitive to the presence of excess of the multidrug resistance drugs colchicine and vinblastine. |
