| First Author | Vartiainen MK | Year | 2007 |
| Journal | Science | Volume | 316 |
| Issue | 5832 | Pages | 1749-52 |
| PubMed ID | 17588931 | Mgi Jnum | J:265632 |
| Mgi Id | MGI:6201987 | Doi | 10.1126/science.1141084 |
| Citation | Vartiainen MK, et al. (2007) Nuclear actin regulates dynamic subcellular localization and activity of the SRF cofactor MAL. Science 316(5832):1749-52 |
| abstractText | Actin, which is best known as a cytoskeletal component, also participates in the control of gene expression. We report a function of nuclear actin in the regulation of MAL, a coactivator of the transcription factor serum response factor (SRF). MAL, which binds monomeric actin, is cytoplasmic in many cells but accumulates in the nucleus upon serum-induced actin polymerization. MAL rapidly shuttles between cytoplasm and nucleus in unstimulated cells. Serum stimulation effectively blocks MAL nuclear export, which requires MAL-actin interaction. Nuclear MAL binds SRF target genes but remains inactive unless actin binding is disrupted. Fluorescence resonance energy transfer analysis demonstrates that the MAL-actin interaction responds to extracellular signals. Serum-induced signaling is thus communicated to nuclear actin to control a transcriptional regulator. |
