| First Author | Cebrat M | Year | 2005 |
| Journal | Eur J Immunol | Volume | 35 |
| Issue | 7 | Pages | 2230-8 |
| PubMed ID | 15971274 | Mgi Jnum | J:100437 |
| Mgi Id | MGI:3588492 | Doi | 10.1002/eji.200526225 |
| Citation | Cebrat M, et al. (2005) Identification of a third evolutionarily conserved gene within the RAG locus and its RAG1-dependent and -independent regulation. Eur J Immunol 35(7):2230-8 |
| abstractText | Recombination-activating gene (RAG)1 and RAG2 encode T and B lymphocyte-specific endonucleases indispensable for rearrangements of antigen-receptor gene segments but also capable of causing deleterious chromosome rearrangements. The mechanisms regulating RAG expression and repression are not clear. Here we identify NWC, a third evolutionarily conserved gene within the RAG locus, and show that it is ubiquitously expressed, with the notable exception of RAG-nonexpressing immature and mature T and B lymphocytes because in lymphocytes it is regulated by the RAG1 promoter and transcribed as RAG1-NWC hybrid mRNA molecules. We also show that in all other cells NWC is controlled by the RAG2 intragenic promoter, which in immature and mature T and B lymphocytes is silent. The possible implications of these findings for understanding the activation and inactivation of RAG genes in lymphocytes and their repression in other cells are discussed. |
