| First Author | Abe R | Year | 1996 |
| Journal | Nucleic Acids Res | Volume | 24 |
| Issue | 11 | Pages | 2011-6 |
| PubMed ID | 8668530 | Mgi Jnum | J:33566 |
| Mgi Id | MGI:81045 | Doi | 10.1093/nar/24.11.2011 |
| Citation | Abe R, et al. (1996) Target specificity of neuronal RNA-binding protein, Mel-N1: direct binding to the 3' untranslated region of its own mRNA. Nucleic Acids Res 24(11):2011-6 |
| abstractText | We have identified cDNAs encoding Mel-N1, the mouse homologue of a human nervous system-specific RNA-binding protein, Hel-N1. Two major mRNA transcripts of Mel-N1 were detected predominantly in the adult mouse brain by Northern blot analysis. To gain insight into the RNA binding specificity of Mel-N1, we performed iterative in vitro RNA selection. The resulting in vitro selected RNAs were found to contain AU-rich sequences as well as a GAAA motif in the majority of clones. By means of in vitro binding assays we demonstrate that this GAAA sequence appears to significantly affect the Mel-N1 RNA-binding efficiency. Our studies further reveal that Mel-N1 can bind to its own 3' untranslated region (3'UTR) as well as to the c-fos 3'UTR, and is localized predominantly in the cytoplasmic region in cells, suggesting that posttranscriptional autoregulation of Mel-N1 gene expression occurs in vivo. |
