| First Author | Kim HW | Year | 2005 |
| Journal | Biochem Biophys Res Commun | Volume | 337 |
| Issue | 1 | Pages | 75-81 |
| PubMed ID | 16171775 | Mgi Jnum | J:101502 |
| Mgi Id | MGI:3604174 | Doi | 10.1016/j.bbrc.2005.08.262 |
| Citation | Kim HW, et al. (2005) Mouse disabled 2 interacting protein 2 functions as a transcriptional repressor through direct binding onto its own promoter. Biochem Biophys Res Commun 337(1):75-81 |
| abstractText | The mDaIP2 protein is a mouse orthologue of human Nostrin (a regulator of eNos). The absence of eNos activity in RA-treated F9 cell implies that the protein plays somehow different role from Nostrin. In this experiment, this protein has been shown to repress the expression of its own gene, via a feedback mechanism which involves binding to the promoter region. Data from cotransfection, DNAP, mDaIP2-silenced F9 cell, and EMSA analyses clearly support the repression activity and direct binding of the protein to the promoter region. The protein contains N-terminal FCH domain and C-terminal SH3 domain. The SH3 domain is known to interact with the proline-rich domain of mDab2, while even no function has been reported about the FCH domain. Here, we report a novel function of mDaIP2 as a transcriptional repressor and suggest the possible association of the FCH domain with transcriptional regulation. |
