| First Author | Horstkorte R | Year | 1999 |
| Journal | Eur J Biochem | Volume | 260 |
| Issue | 3 | Pages | 923-7 |
| PubMed ID | 10103025 | Mgi Jnum | J:53895 |
| Mgi Id | MGI:1333609 | Doi | 10.1046/j.1432-1327.1999.00253.x |
| Citation | Horstkorte R, et al. (1999) Tissue expression and amino acid sequence of murine UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase. Eur J Biochem 260(3):923-7 |
| abstractText | Neuraminic acids are widely expressed as terminal carbohydrates on glycoconjugates and are involved in a variety of biological functions. The key enzyme of N-acetylneuraminic acid synthesis is UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase, which catalyses the first two steps of neuraminic acid biosynthesis in the cytosol. In this study we report the complete amino acid sequence of the mouse UDP-N acetylglucosamine-2-epimerase/N-acetylmannosamine kinase. The ORF of 2166 bp encodes 722 amino acids and a protein with a predicted molecular mass of 79.2 kDa. Northern blot analysis and in situ hybridization revealed that UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase is expressed at early stages during development and in all tissues investigated with a maximal expression in the liver. |
