| First Author | Yamasaki N | Year | 1987 |
| Journal | Eur J Immunol | Volume | 17 |
| Issue | 2 | Pages | 247-53 |
| PubMed ID | 3493908 | Mgi Jnum | J:64946 |
| Mgi Id | MGI:1891493 | Doi | 10.1002/eji.1830170215 |
| Citation | Yamasaki N, et al. (1987) Sequence analysis of a cDNA clone of a gene encoding a component of a putative phosphorylcholine-specific T suppressor factor and functional property of its gene product. Eur J Immunol 17(2):247-53 |
| abstractText | We have isolated a putative phosphorylcholine (PC)-T cell suppressor factor (TsF) cDNA clone, p6-5, from a cDNA library of a T hybridoma which constitutively secretes a PC-TsF in vitro [8]. In the present study, we determined the nucleotide sequence of the p6-5 gene and found that the p6-5 sequence is 86% homologous to rat preproelastase 1 gene, one of the serine protease genes. An oligopeptide (14 mer, TsF14) deduced from the p6-5 sequence was synthesized and antisera against TsF14 were prepared in rabbits. Anti-TsF14-conjugated Sepharose 4B specifically absorbed the PC-TsF activity from the culture supernatant of PC-TsF-secreting T hybridomas. In contrast, the binding molecule eluted from the anti-TsF14-conjugated Sepharose suppressed the antibody response PC specifically. These results indicated that the p6-5 polypeptide is a component of the PC-TsF molecule. PMID- 0003493908 |
