| First Author | Metz RP | Year | 2006 |
| Journal | J Biol Chem | Volume | 281 |
| Issue | 16 | Pages | 10839-48 |
| PubMed ID | 16484282 | Mgi Jnum | J:112724 |
| Mgi Id | MGI:3663230 | Doi | 10.1074/jbc.M508858200 |
| Citation | Metz RP, et al. (2006) Differential transcriptional regulation by mouse single-minded 2s. J Biol Chem 281(16):10839-48 |
| abstractText | Single-minded 1 and 2 are unique members of the basic helix-loop-helix Per-Arnt-Sim family as they are transcriptional repressors. Here we report the identification and transcriptional characterization of mouse Sim2s, a splice variant of Sim2, which is missing the carboxyl Pro/Ala-rich repressive domain. Sim2s is expressed at high levels in kidney and skeletal muscle; however, the ratio of Sim2 to Sim2s mRNA differs between these tissues. Similar to full-length Sim2, Sim2s interacts with Arnt and to a lesser extent, Arnt2. The effects of Sim2s on transcriptional regulation through hypoxia, dioxin, and central midline response elements are different than that of full-length Sim2. Specifically, Sim2s exerts a less repressive effect on hypoxia-induced gene expression than full-length Sim2, but is just as effective as Sim2 at repressing TCDD-induced gene expression from a dioxin response element. Interestingly, Sim2s bind to and activates expression from a central midline response element-controlled reporter through an Arnt transactivation domain-dependent mechanism. The differences in expression pattern, protein interactions, and transcriptional activities between Sim2 and Sim2s may reflect differential roles each isoform plays during development or in tissue-specific effects on other protein-mediated pathways. |
