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Allele : Col1a1<tm3Nki> collagen, type I, alpha 1; targeted mutation 3, The Netherlands Cancer Institute
Primary Identifier  MGI:5443866 Allele Type  Targeted
Attribute String  Conditional ready, No functional change Gene  Col1a1
Transmission  Germline Strain of Origin  Not Specified
Is Recombinase  false Is Wild Type  false
description  Parental ES cells contain Cdkn2atm1.1Brn Nf2tm2Gth Trp53tm1Brn mice.
molecularNote  The ES cells were targeted in the 3' UTR of the Col1a1 locus with a Flp-in construct containing a frt flanked PGK-Neo cassette followed by a Hygromicin gene lacking a proper ATG. Correctly targeted ES cells were selected on basis of Neomycin resistance. The targeted Col1a1 locus can later be used as a docking site for the introduction of a plasmid, minimally containing the desired transgene sequence and an frt site preceded by a constitutive promoter and an ATG initiation codon.
  • mutations:
  • Insertion
  • synonyms:
  • Col1a1<tm3(frt-PGK-neo-frt)Nki>,
  • Col1a1<tm3(frt-PGK-neo-frt)Nki>,
  • Col1a1<tm3(frt-PGK-neo-frt-deltaATGHygro)Nki>,
  • Col1a1<tm3(frt-PGK-neo-frt-deltaATGHygro)Nki>
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1 Feature

Trail: Allele

Genome

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0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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