Primary Identifier | MGI:7570125 | Allele Type | Targeted |
Attribute String | Conditional ready, Null/knockout, Reporter | Gene | Crisp2 |
Inheritance Mode | Not Specified | Transmission | Germline |
Strain of Origin | C57BL/6NSlc-Tg(CAG-EGFP,Acr-EGFP)2Osb | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Using targeting vector (PG00061_Y_6_A09), the L1L2_Bact_P cassette was inserted at position 41095297 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 41096404. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |