| Primary Identifier | MGI:6119758 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout | Gene | Zfand2b |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A FRT-flanked trapping element consisting of a splice acceptor sequence followed by a neomycin resistance gene and a loxP site were inserted into introns 1-2. A second loxP site was inserted into introns 8-9 so that exons 2-8 were floxed. qRT-PCR analysis demonstrated the loss of mRNA expression in liver, muscle and bone marrow tissue from homozygous mutant mice. Western blot analysis confirmed absence of encoded protein. |
