| First Author | Oertle T | Year | 2003 |
| Journal | J Mol Biol | Volume | 325 |
| Issue | 2 | Pages | 299-323 |
| PubMed ID | 12488097 | Mgi Jnum | J:81211 |
| Mgi Id | MGI:2448324 | Doi | 10.1016/s0022-2836(02)01179-8 |
| Citation | Oertle T, et al. (2003) Genomic Structure and Functional Characterisation of the Promoters of Human and Mouse nogo/rtn4. J Mol Biol 325(2):299-323 |
| abstractText | The reticulon-family member Nogo-A is a potent neurite growth inhibitory protein in vitro and may play a role in the restriction of axonal regeneration after injury and of structural plasticity in the CNS of higher vertebrates. Of the three major isoforms of Nogo, Nogo-A is mostly expressed in the brain, Nogo-B is found in a ubiquitous pattern, and Nogo-C is most highly expressed in muscle. Seven additional splice-variants derived both from differential splicing and differential promoter usage have been identified. Analysis of the TATA-less Nogo-A/B promoter (P1) shows that conserved GC-boxes and a CCAAT-box within the first 500bp upstream of the transcription start are responsible for its regulation. No major differences in the methylation status of the P1 CpG-island in tissues expressing or not expressing Nogo-A/B could be detected, suggesting that silencer elements are involved in the regulation. The specific expression pattern of Nogo-A/B is due to differential splicing. The basal Nogo-C promoter (P2) is regulated by a proximal and a distal element. The 5'UTR of Nogo-C harbours a negative control element. These data may help to identify factors that can modulate Nogo transcription, thus offering an alternative approach for Nogo neutralisation. |
