| First Author | Franke K | Year | 1998 |
| Journal | Biochim Biophys Acta | Volume | 1398 |
| Issue | 3 | Pages | 232-42 |
| PubMed ID | 9655912 | Mgi Jnum | J:49367 |
| Mgi Id | MGI:1277408 | Doi | 10.1016/s0167-4781(98)00062-1 |
| Citation | Franke K, et al. (1998) Expression of murine H1 histone genes during postnatal development. Biochim Biophys Acta 1398(3):232-42 |
| abstractText | Murine genes encoding the seven H1 histone isoforms H1.1-H1.5, H1(o) and H1t have been isolated and sequenced. We have established expression patterns of these genes in several tissues during postnatal development. For that analysis, RNase protection assay rather than Northern blot hybridization was used, since the sequences of these genes are highly similar and would cross-hybridize under Northern blot conditions. Expression patterns of H1.1 to H1.5 and H1(o) were determined in tissues of animals at days 5, 9 and 20 after birth and of adult mice. In addition, RNA was analyzed in three mouse cell lines (NIH3T3, P19, TM4). Transcription of the subtype genes H1.2 and H1.4 was found in all tissues and cell lines studied. The most varied expression patterns were obtained with the H1.1 subtype. H1.1 mRNA was found at high concentrations in thymus and spleen throughout development and in testis beginning with a low expression in 5-day-old animals and increasing levels in testis RNA from 9- and 20-day-old and adult mice. H1(o) mRNA was found primarily in highly differentiated tissues with concentrations decreasing from 5-day-old to adult animals. |
