| First Author | Freire-Moar J | Year | 1995 |
| Journal | Biochim Biophys Acta | Volume | 1254 |
| Issue | 1 | Pages | 112-6 |
| PubMed ID | 7811740 | Mgi Jnum | J:22314 |
| Mgi Id | MGI:70194 | Doi | 10.1016/0005-2760(94)00199-9 |
| Citation | Freire-Moar J, et al. (1995) Cloning and characterization of a murine macrophage lipoxygenase. Biochim Biophys Acta 1254(1):112-6 |
| abstractText | We have isolated a murine macrophage cDNA encoding a 12-lipoxygenase, that represents the homolog of the human 15-lipoxygenase. The predicted amino acid sequence of this lipoxygenase is highly similar to the rat 12-lipoxygenase isolated from brain and human 15-lipoxgenase. The recombinant enzyme expressed in Cos-7 cells oxidizes arachidonic acid to 12- and 15-HETE with a profile similar to that obtained from peritoneal macrophages. A polyclonal antibody generated against a putative peptide recognizes a 75 kDa protein in cell extracts from mouse peritoneal macrophages and transfected Cos-7 cells. The lipoxygenase cDNA hybridizes to a 2.5 kb mRNA present in peritoneal macrophages, lung, spleen, heart and liver. RT-PCR analysis indicates that the same lipoxygenase is expressed in mouse reticulocytes. A partial genomic clone for this lipoxygenase has also been characterized. Southern blot analysis of mouse genomic DNA indicates that this is a single copy gene. |
