| First Author | VanBuren V | Year | 2002 |
| Journal | Genome Res | Volume | 12 |
| Issue | 12 | Pages | 1999-2003 |
| PubMed ID | 12466305 | Mgi Jnum | J:87348 |
| Mgi Id | MGI:3029767 | Doi | 10.1101/gr.633802 |
| Citation | VanBuren V, et al. (2002) Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set. Genome Res 12(12):1999-2003 |
| abstractText | A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ~120,000 cDNA clones, which were initially re-arrayed into a set of ~11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers. |
