| Primary Identifier | MGI:5469319 | Allele Type | Chemically and radiation induced |
| Gene | mt-Nd6 | Transmission | Germline |
| Strain of Origin | 129S/SvEv-Gpi1<c> | Is Recombinase | false |
| Is Wild Type | false |
| description | ES cell line = CC9.3.1 |
| molecularNote | Treatment with psoralen and UV light was used to generate a clone (LT13) homoplastic for mitochondria with a G-to-A mutation at position 13997 that results in the amino acid substitution of proline with leucine at position 25 (p.P25L). This mutation is equivalent to the human mutation g.14600G>A (p.P25L) associated with optic atrophy and Leigh syndrome. Rhodamine-6G treatment was used to deplete ES cells of endogenous mitochondria. |
