| First Author | Froussard P | Year | 1988 |
| Journal | J Immunol | Volume | 141 |
| Issue | 4 | Pages | 1358-64 |
| PubMed ID | 3135330 | Mgi Jnum | J:9264 |
| Mgi Id | MGI:57727 | Doi | 10.4049/jimmunol.141.4.1358 |
| Citation | Froussard P, et al. (1988) p55 IL-2 receptor mRNA precursors in murine T lymphocyte nuclei. J Immunol 141(4):1358-64 |
| abstractText | An unusual family of cDNA clones homologous to human p55 IL-2R sequences was isolated from the murine HT-2 Th cell line. These clones were mapped, partially sequenced, and compared with previously published human and mouse IL-2R sequences. They appear to consist of various combinations of exons and introns, suggesting that they are derived from p55 IL-2R mRNA precursors. The configuration of exons in the splicing intermediates indicates that the murine and human gene organizations are similar and that the 3' end of intron 3 is well conserved between the two species. RNA mapping experiments using nuclear, cytoplasmic, and total RNA and probes derived from various parts of the p55 IL-2R gene support and extend the sequence data. They indicate that detectable amounts of immature p55 IL-2R mRNA are found specifically in the cell nucleus of the HT-2 cell line. Similar data were obtained for the Th cell clone 52.3 and the cytotoxic T cell line CTLL. All these results indicate that the T cell nucleus contains significant amounts of immature p55 IL-2R mRNA. |
