| First Author | Chiasson VL | Year | 2012 |
| Journal | Kidney Int | Volume | 82 |
| Issue | 8 | Pages | 857-66 |
| PubMed ID | 22495293 | Mgi Jnum | J:198179 |
| Mgi Id | MGI:5495623 | Doi | 10.1038/ki.2012.104 |
| Citation | Chiasson VL, et al. (2012) Endothelial cell transforming growth factor-beta receptor activation causes tacrolimus-induced renal arteriolar hyalinosis. Kidney Int 82(8):857-66 |
| abstractText | Arteriolar hyalinosis is a common histological finding in renal transplant recipients treated with the calcineurin inhibitor tacrolimus; however, the pathophysiologic mechanisms remain unknown. In addition to increasing transforming growth factor (TGF)-beta levels, tacrolimus inhibits calcineurin by binding to FK506-binding protein 12 (FKBP12). FKBP12 alone also inhibits TGF-beta receptor activation. Here we tested whether tacrolimus binding to FKBP12 removes an inhibition of the TGF-beta receptor, allowing ligand binding, ultimately leading to receptor activation and arteriolar hyalinosis. We found that specific deletion of FKBP12 from endothelial cells was sufficient to activate endothelial TGF-beta receptors and induce renal arteriolar hyalinosis in these knockout mice, similar to that induced by tacrolimus. Tacrolimus-treated and knockout mice exhibited significantly increased levels of aortic TGF-beta receptor activation as evidenced by SMAD2/3 phosphorylation, along with increased collagen and fibronectin expression compared to controls. Treatment of isolated mouse aortas with tacrolimus increased TGF-beta receptor activation and collagen and fibronectin expression. These effects were independent of calcineurin, absent in endothelial denuded aortic rings, and could be prevented by the small molecule TGF-beta receptor inhibitor SB-505124. Thus, endothelial cell TGF-beta receptor activation is sufficient to cause vascular remodeling and renal arteriolar hyalinosis. |
