| First Author | Janardhan KS | Year | 2012 |
| Journal | Cell Tissue Res | Volume | 348 |
| Issue | 1 | Pages | 177-87 |
| PubMed ID | 22350844 | Mgi Jnum | J:325794 |
| Mgi Id | MGI:6872558 | Doi | 10.1007/s00441-011-1300-9 |
| Citation | Janardhan KS, et al. (2012) Integrin beta3 is not critical for neutrophil recruitment in a mouse model of pneumococcal pneumonia. Cell Tissue Res 348(1):177-87 |
| abstractText | Streptococcus pneumoniae is one of the most common causes of bacterial pneumonias in humans. Neutrophil migration into lungs infected with S. pneumoniae is central to the host defense but the mechanisms of neutrophil recruitment, as mediated by S. pneumoniae, into lungs are incompletely understood. Therefore, we have assessed the role of integrin alphavbeta3 by evaluating its subunit beta3 in a mouse model of lung inflammation induced by S. pneumonia. Integrin subunit beta3 knockout (beta3(-/-)) and wild-type (WT) mice were intratracheally instilled with either S. pneumoniae or saline. Other groups of WT mice were treated intraperitoneally with 25 mug or 50 mug of antibody against integrin beta3 or with isotype-matched antibody at 1 h before instillation of S. pneumoniae. Mice were killed 24 h after infection. Flow cytometry confirmed the absence or presence of integrin subunit beta3 on peripheral blood neutrophils in beta3(-/-) or WT mice, respectively. Neutrophil numbers in bronchoalveolar lavage (BAL) from infected beta3(-/-) and WT mice showed no differences. Neutrophil numbers in BAL of infected WT mice treated with beta3 antibody were lower compared with those without antibody but similar to those of mice administered isotype-matched antibody. Many neutrophils were present in the perivascular spaces of the lungs in beta3(-/-) mice. Lungs from infected beta3(-/-) mice had negligible mitogen-activated protein kinase expression compared with those of infected WT mice. Thus, integrin beta3 or its heterodimer alphavbeta3 is not critical for neutrophil migration into lungs infected with S. pneumoniae. |
