First Author | Arai S | Year | 2007 |
Journal | FEBS Lett | Volume | 581 |
Issue | 29 | Pages | 5649-57 |
PubMed ID | 18022393 | Mgi Jnum | J:127713 |
Mgi Id | MGI:3764736 | Doi | 10.1016/j.febslet.2007.11.022 |
Citation | Arai S, et al. (2007) Novel homeodomain-interacting protein kinase family member, HIPK4, phosphorylates human p53 at serine 9. FEBS Lett 581(29):5649-57 |
abstractText | We describe here the cloning and characterization of a novel mouse homeodomain-interacting protein kinase (HIPK)-like gene, Hipk4. Hipk4 is expressed in lung and in white adipose tissue and encodes a 616 amino acid protein that includes a serine/threonine kinase domain. We demonstrate that HIPK4 could phosphorylate human p53 protein at serine 9, both in vitro and in vivo. Among known p53-responsive promoters, activity of the human survivin promoter, which is repressed by p53, was decreased by HIPK4 in p53 functional A549 cells. Human BCL2-associated X protein-promoter activity was not affected. These findings suggest that phosphorylation of p53 at serine 9 is important for p53 mediated transcriptional repression. |