| First Author | Ajjappala BS | Year | 2009 |
| Journal | J Immunol | Volume | 182 |
| Issue | 2 | Pages | 1050-60 |
| PubMed ID | 19124748 | Mgi Jnum | J:143486 |
| Mgi Id | MGI:3827029 | Doi | 10.4049/jimmunol.182.2.1050 |
| Citation | Ajjappala BS, et al. (2009) 14-3-3gamma is stimulated by IL-3 and promotes cell proliferation. J Immunol 182(2):1050-60 |
| abstractText | IL-3 plays important roles in the growth and survival of hematopoietic progenitor cells, processes modeled in studies of the IL-3-dependent cell line Ba/F3. To gain insights into molecular mechanisms governing cell fate, we examined the patterns of proteins up-regulated following stimulation of Ba/F3 cells with IL-3. Through two-dimensional electrophoresis and proteomics-based approaches, we identified 11 proteins. Of these, expression of 14-3-3gamma was significantly increased by IL-3 stimulation at both the transcriptional and translational levels. 14-3-3gamma overexpression in Ba/F3 cells abrogated dependence on IL-3 and was associated with activation of PI3K and MAPK signaling cascades, suggesting that the functions of 14-3-3gamma in normal hematopoietic progenitors are to promote survival and growth through the activation of distinct signaling pathways. Additionally, the up-regulation of Bax and Bad was seen with the ablation of 14-3-3gamma, resulting in cell death. These results indicate that deregulated expression of 14-3-3gamma may contribute to malignant transformation, possibly providing a new target for therapeutic intervention in hematopoietic neoplasms. |
