| First Author | Yu J | Year | 2016 |
| Journal | Development | Volume | 143 |
| Issue | 16 | Pages | 2930-45 |
| PubMed ID | 27471256 | Mgi Jnum | J:302718 |
| Mgi Id | MGI:6509510 | Doi | 10.1242/dev.134247 |
| Citation | Yu J, et al. (2016) Protein synthesis and degradation are essential to regulate germline stem cell homeostasis in Drosophila testes. Development 143(16):2930-45 |
| abstractText | The homeostasis of self-renewal and differentiation in stem cells is controlled by intrinsic signals and their niche. We conducted a large-scale RNA interference (RNAi) screen in Drosophila testes and identified 221 genes required for germline stem cell (GSC) maintenance or differentiation. Knockdown of these genes in transit-amplifying spermatogonia and cyst cells further revealed various phenotypes. Complex analysis uncovered that many of the identified genes are involved in key steps of protein synthesis and degradation. A group of genes that are required for mRNA splicing and protein translation contributes to both GSC self-renewal and early germ cell differentiation. Loss of genes in the protein degradation pathway in cyst cells leads to testis tumors consisting of overproliferated germ cells. Importantly, in the Cullin 4-RING E3 ubiquitin ligase (CRL4) complex, we identified multiple proteins that are crucial to GSC self-renewal: pic/DDB1, a CRL4 linker protein, is not only required for GSC self-renewal in flies but also for maintenance of spermatogonial stem cells (SSCs) in mice. |
