| First Author | Suzuki-Utsunomiya K | Year | 2007 |
| Journal | Biochem Biophys Res Commun | Volume | 354 |
| Issue | 2 | Pages | 491-7 |
| PubMed ID | 17239822 | Mgi Jnum | J:118328 |
| Mgi Id | MGI:3699441 | Doi | 10.1016/j.bbrc.2006.12.229 |
| Citation | Suzuki-Utsunomiya K, et al. (2007) ALS2CL, a novel ALS2-interactor, modulates ALS2-mediated endosome dynamics. Biochem Biophys Res Commun 354(2):491-7 |
| abstractText | ALS2, the causative gene product for a number of recessive motor neuron diseases, is a guanine-nucleotide exchange factor for Rab5, and acts as a modulator for endosome dynamics. Recently, we have identified a novel ALS2 homolog, ALS2CL, which is highly homologous to the C-terminal half of ALS2. In this study, we investigate the molecular features of ALS2CL and its functional relationship with ALS2. A majority of ALS2CL is present as a homo-dimeric form, which can interact with the ALS2-oligomer, resulting in the formation of the large ALS2/ALS2CL heteromeric complex. In cultured cells, overexpressed ALS2CL is colocalized with ALS2 onto membranous compartments. Further, ALS2CL dominantly suppresses the endosome enlargement induced by a constitutively active form of ALS2, and results in an extensive perinuclear tubulo-membranous phenotype, which are dependent upon the ALS2CL-ALS2 interaction. Collectively, ALS2CL is a novel ALS2-interacting protein and is implicated in ALS2-mediated endosome dynamics. |
