| First Author | Valdivia RP | Year | 1996 |
| Journal | Exp Anim | Volume | 45 |
| Issue | 4 | Pages | 395-8 |
| PubMed ID | 8902505 | Mgi Jnum | J:38589 |
| Mgi Id | MGI:85968 | Doi | 10.1538/expanim.45.395 |
| Citation | Valdivia RP, et al. (1996) Typing of X chromosomes bearing Tabby allele in mouse preimplantation embryos by detection of a microsatellite marker. Exp Anim 45(4):395-8 |
| abstractText | Tabby (Ta) is a semidominat allele of the locus on the mouse X chromosome, which causes a characteristic coat pattern and developmental defects in endocrinic glands. To establish a method for identifying the X chromosome bearing the Ta allele in early preimplantation embryos, we performed PCR amplification of an X chromosomal microsatellite marker locus from preimplantation embryos obtained from mating between XO female and XY male mice. The microsatellite marker locus was shown to be polymorphic between X chromosomes bearing the Ta and wild-type alleles. The amplification of the marker locus from early preimplantation embryos demonstrated that the Ta locus can be correctly typed by this method from embryos as little as 2 cells. |
