| First Author | Dong M | Year | 2006 |
| Journal | Science | Volume | 312 |
| Issue | 5773 | Pages | 592-6 |
| PubMed ID | 16543415 | Mgi Jnum | J:108334 |
| Mgi Id | MGI:3623705 | Doi | 10.1126/science.1123654 |
| Citation | Dong M, et al. (2006) SV2 is the protein receptor for botulinum neurotoxin A. Science 312(5773):592-6 |
| abstractText | How the widely used botulinum neurotoxin A (BoNT/A) recognizes and enters neurons is poorly understood. We found that BoNT/A enters neurons by binding to the synaptic vesicle protein SV2 (isoforms A, B, and C). Fragments of SV2 that harbor the toxin interaction domain inhibited BoNT/A from binding to neurons. BoNT/A binding to SV2A and SV2B knockout hippocampal neurons was abolished and was restored by expressing SV2A, SV2B, or SV2C. Reduction of SV2 expression in PC12 and Neuro-2a cells also inhibited entry of BoNT/A, which could be restored by expressing SV2 isoforms. Finally, mice that lacked an SV2 isoform (SV2B) displayed reduced sensitivity to BoNT/A. Thus, SV2 acts as the protein receptor for BoNT/A. |
